FastQCFastQC Report
Thu 29 Aug 2019
H5LJCBGXC_n01_mb236.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5LJCBGXC_n01_mb236.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28582104
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC5154721.8034781484246225TruSeq Adapter, Index 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG618150.065.5955443
CTCGTAT589450.065.5446842
CGTATGC622300.065.4224544
TCTCGTA579250.065.3937641
TATGCCG623400.065.2059546
ATGCCGT623850.065.0611147
CACGATC575050.064.9149336
ACGATCT574750.064.8331237
ATCACGA616950.064.8178234
TCACGAT603600.064.7374935
CGATCTC576800.064.66337638
GATCTCG579800.064.41550439
GCCGTCT630250.064.2572149
CATCACG632750.064.25574533
TGCCGTC632350.064.1489848
ATCTCGT583550.064.0675340
CCGTCTT633500.063.98336850
CGTCTGA650550.063.71669816
ACACGTC657400.063.34693513
CGTCTTC641950.063.08046751
Produced by FastQC (version 0.11.5)