FastQCFastQC Report
Thu 29 Aug 2019
H5LJCBGXC_n01_mb235.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5LJCBGXC_n01_mb235.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39775585
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC4639811.1664969855251657TruSeq Adapter, Index 12 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG560800.065.5825243
CGTATGC565950.065.2640444
CTCGTAT532100.065.0713842
TCTCGTA529450.065.0533441
ATGCCGT568350.064.68394547
TATGCCG572600.064.3043746
AATCTCG539150.063.83196339
TGCCGTC578150.063.53436348
GCCGTCT577650.063.10463349
CCGTCTT583100.062.63541850
ATCTCGT550150.062.5877240
CGTCTTC591900.061.59104551
CGTCTGA623900.060.68105716
ACGTCTG631000.059.99235515
ACACGTC632550.059.98088513
GTATGCC617350.059.8755745
CACGTCT649450.058.3948814
ATCGGAA680550.055.9054072
GTAATCT617350.055.86861437
CACACGT683350.055.627812
Produced by FastQC (version 0.11.5)