FastQCFastQC Report
Thu 29 Aug 2019
H5LJCBGXC_n01_mb234.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5LJCBGXC_n01_mb234.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32984563
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC2271690.6887130807220335TruSeq Adapter, Index 7 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC284850.062.19911244
TCGTATG276750.062.19839543
CTCGTAT267450.062.0292142
TCTCGTA266800.061.90484641
ATGCCGT289600.060.69815447
TATGCCG292300.060.68575746
TGCCGTC296100.059.3371248
ATCTCGT283750.059.05263540
GCCGTCT290050.059.0423349
CCGTCTT294650.058.3108150
CGTCTGA335000.057.7352516
ACACGTC338750.057.31994613
CATCTCG294050.057.18646239
ACGTCTG341600.056.70163715
CGTCTTC300400.056.41918651
GATCGGA355350.055.359881
ATCGGAA357750.055.1753122
CACGTCT354350.054.76909314
GTATGCC328450.054.32621845
TCGGAAG372850.052.790593
Produced by FastQC (version 0.11.5)