FastQCFastQC Report
Thu 29 Aug 2019
H5LJCBGXC_n01_mb233.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5LJCBGXC_n01_mb233.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33243057
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC2144510.6451001181991175TruSeq Adapter, Index 6 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT262950.062.29150442
TCGTATG265750.062.2571743
TCTCGTA264000.062.12329541
CGTATGC267400.061.99081444
CGCCAAT270050.061.65124533
ATGCCGT270900.060.99930647
TATCTCG270500.060.6646639
TATGCCG274550.060.30000746
ACGCCAA280600.059.56038332
GTCACGC281250.059.43312529
TGCCGTC277800.059.41691648
ATCTCGT278850.058.84809540
GCCGTCT279900.058.7835649
CCGTCTT281250.058.56406450
TCACGCC294550.056.75010330
CGTCTTC291150.056.72131751
AGTCACG297900.056.1347528
CACGCCA301300.055.64135731
CGTCTGA306150.055.4133616
ACACGTC315550.054.02600513