FastQCFastQC Report
Thu 29 Aug 2019
H5LJCBGXC_n01_mb232.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5LJCBGXC_n01_mb232.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27630371
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC1434800.5192836534840592TruSeq Adapter, Index 5 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG202600.062.45221743
CGTATGC207150.062.44907844
CTCGTAT178000.061.79921342
TCTCGTA176450.061.60817741
ATGCCGT209200.061.57232747
TATGCCG213250.060.85968446
TGCCGTC214100.059.79785548
GCCGTCT213200.059.1802349
GATCTCG183400.058.90453339
CCGTCTT216950.058.36743550
ATCTCGT187450.057.65062740
CGTCTGA239050.057.63009316
CGTCTTC221650.056.6465351
ACACGTC245000.056.45376613
ACGTCTG246050.056.0745715
CACGTCT256950.053.81727614
GTATGCC245350.053.0683945
ATCGGAA270300.051.6108742
GATCGGA270250.051.3317531
CACACGT278950.049.7057512
Produced by FastQC (version 0.11.5)