FastQCFastQC Report
Thu 29 Aug 2019
H5LJCBGXC_n01_mb231.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5LJCBGXC_n01_mb231.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24280284
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC2335600.9619327352184184TruSeq Adapter, Index 4 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT273850.063.2645642
TCTCGTA271700.063.21113241
TCGTATG292650.063.17310743
CGTATGC295550.062.6361344
ATGCCGT294750.062.5375347
AATCTCG273050.062.42995539
TATGCCG298400.061.87368846
TGCCGTC298750.061.6469948
CCGTCTT302350.060.85534750
GCCGTCT302000.060.82131649
CGTCTTC305150.060.337251
CGTCTGA321900.059.1500216
ACACGTC325700.058.8646313
ATCTCGT292300.058.49821540
ACGTCTG326250.058.3284515
CACGTCT339250.056.29907614
GTATGCC335400.055.13151645
ACCAATC316250.053.72934336
CACACGT357950.053.66626412
GACCAAT338750.053.4768735
Produced by FastQC (version 0.11.5)