FastQCFastQC Report
Thu 29 Aug 2019
H5LJCBGXC_n01_mb230.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5LJCBGXC_n01_mb230.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25738898
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC2784201.0817090925959612TruSeq Adapter, Index 2 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA313950.064.9722341
TCGTATG320200.064.8978743
CTCGTAT315000.064.8556542
CGATGTA339600.064.6575734
ACCGATG341400.064.5058432
CACCGAT342900.064.3451431
TATCTCG319950.064.32683639
CGTATGC328950.064.2569144
ATGCCGT327650.064.120147
TATGCCG329850.064.0603746
CCGATGT343750.063.89734333
TGCCGTC328300.063.84846548
GTCACCG350550.062.8999129
GCCGTCT327950.062.81735649
CCGTCTT331300.062.53103650
TCACCGA353800.062.39245230
CGTCTTC331050.061.8867351
ATCTCGT337300.060.9661340
ACACGTC368050.060.8198113
CGTCTGA369900.060.30969616
Produced by FastQC (version 0.11.5)