FastQCFastQC Report
Sat 20 Jan 2018
H5L5MBCX2_l02n02_lib3_dropseq.352000000b9c4d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5L5MBCX2_l02n02_lib3_dropseq.352000000b9c4d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123378996
Sequences flagged as poor quality0
Sequence length33
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACGTACTCTGCGTTGATACCACTGCTTCCGCG5835350.4729613782884082No Hit
ACTTAAATGTGACCTAGTAGATAAAATCATCAT5040160.4085103756234165No Hit
GTCTAAGACATGTATCACGAATTGAAAATGATA4984150.40397070502989013No Hit
CACGTACTCTGCGTTGATACCACTGCTTCCGCG4629300.37520973180880807No Hit
GGTGCATATGGTGCCAACCCCCCTGGCCCCCCC3251830.26356431041147393No Hit
TACGTACTCTGCGTTGATACCACTGCTTCCGCG2821320.22867101301424111No Hit
GTACTCTGCGTTGATACCACTGCTTCCGCGGAC2574110.2086343772808785No Hit
AACGTACTCTGCGTTGATACCACTGCTTCCGCG2551080.20676777107182814No Hit
CCCCAAAAGGGGGGTGGTGCTGGTGCAATTGCA2424280.19649049502720867No Hit
GTGTTGGAGGAACTCAAACAAAACAAGCAAAAA2158260.17492928861246368No Hit
AGTCTAAGACATGTATCACGAATTGAAAATGAT2146440.1739712649307018No Hit
CCCAAAAGGGGGGTGGTGCTGGTGCAATTGCAA2145810.17392020275477035No Hit
GTCTGATACGCCGCCCGCCACGCCCATCGTGTT2063720.16726672017982705No Hit
AGTTATATCAATGCAAACAAAAAAATCTAAAAA2004550.1624709281959143No Hit
GTGCATATGGTGCCAACCCCCCTGGCCCCCCCA1956460.1585731820998122No Hit
CTCTCGAATTGTGTCCGTTGGTAGCCGAAGTTA1840360.14916315253529863No Hit
CATAAAATACACTAAAAAAAGAGAAGACTTACT1814670.14708095047231542No Hit
GTGCTGGTGCAATTGCAAAGGCACAGGAGCACA1780250.14429117254285323No Hit
AAGTTATATCAATGCAAACAAAAAAATCTAAAA1777820.144094218435689No Hit
GTACGTACTCTGCGTTGATACCACTGCTTCCGC1776920.14402127247007263No Hit
CCATAGAGACGTTTAAGGCAAATTCTAAATGAA1743120.14128174620581285No Hit
CCCCTGGCCCCCCCAAAAGGGGGGTGGTGCTGG1640050.13292781212127874No Hit
GAGCACAGGAGCAGCTAAATAAACAAGAAAGCA1545910.12529766411780496No Hit
GACATGTATCACGAATTGAAAATGATAATGACA1544720.1252012133410455No Hit
CGTACTCTGCGTTGATACCACTGCTTCCGCGGA1492060.12093306384175796No Hit
CTCTGAAGCAAAAGCCAAGGCCGGAGCAGGTGC1487670.12057724963169582No Hit
CTCGAATTGTGTCCGTTGGTAGCCGAAGTTACG1479420.1199085782802123No Hit
GTGCCAACCCCCCTGGCCCCCCCAAAAGGGGGG1423920.1154102437338686No Hit
GGCACAGGAGCACAGGAGCAGCTAAATAAACAA1404820.11386216824134311No Hit
GCACAGGAGCAGCTAAATAAACAAGAAAGCAAA1364960.11063147247526636No Hit
GCTAAATAAACAAGAAAGCAAAATCAACAACCG1296090.10504948508415483No Hit
CTCTCTCACTCTCTGAAGCAAAAGCCAAGGCCG1290130.10456642068962857No Hit
GTATAAACCAAAAACAATTGTAACGAGAAAACA1271830.10308318605542877No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA352700.021.0735661
TTCCGCG2349200.019.00214227
CGCGGAC406200.017.624227
GTATCAA1366800.017.1516531
CTTCCGC2831150.015.75214426
GACCTAG974450.014.94530911
TGACCTA996800.014.68469610
GTGACCT1000600.014.6290059
GTGTTGG489350.014.2838331
GGAACTC454600.014.2688769
GTTGGAG486150.014.0187973
TTGGAGG480650.013.8417614
CCTAGTA1057200.013.83167213
TGTTGGA504500.013.6484742
ACCTAGT1070750.013.60495612
CGCCCGC439100.013.49353212
CCCGCCA447700.013.19512114
GCCCGCC450550.013.19255313
TGTGACC1139100.013.0375548
AGGAACT511700.012.9773858
Produced by FastQC (version 0.11.2)