FastQCFastQC Report
Sat 20 Jan 2018
H5L5MBCX2_l02n02_lib1212_dropseq.352000000b9c23.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5L5MBCX2_l02n02_lib1212_dropseq.352000000b9c23.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33956229
Sequences flagged as poor quality0
Sequence length33
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACGTACTCTGCGTTGATACCACTGCTTCCGCG1276150.3758220619845625No Hit
CACGTACTCTGCGTTGATACCACTGCTTCCGCG941170.27717153162089936No Hit
GTCTAAGACATGTATCACGAATTGAAAATGATA928440.27342258764952965No Hit
GGTGCATATGGTGCCAACCCCCCTGGCCCCCCC882010.25974910229283704No Hit
ACTTAAATGTGACCTAGTAGATAAAATCATCAT690750.20342364872141722No Hit
TACGTACTCTGCGTTGATACCACTGCTTCCGCG634990.18700250843519756No Hit
AACGTACTCTGCGTTGATACCACTGCTTCCGCG617190.18176046580437422No Hit
CTCTCGAATTGTGTCCGTTGGTAGCCGAAGTTA589730.17367358430760968No Hit
GTCTGATACGCCGCCCGCCACGCCCATCGTGTT536190.15790622686635786No Hit
GTGCATATGGTGCCAACCCCCCTGGCCCCCCCA516000.15196033693847452No Hit
CTCGAATTGTGTCCGTTGGTAGCCGAAGTTACG490520.1444565590601948No Hit
CCCCAAAAGGGGGGTGGTGCTGGTGCAATTGCA488080.14373798692428422No Hit
GTACTCTGCGTTGATACCACTGCTTCCGCGGAC466530.1373915813796638No Hit
CCCAAAAGGGGGGTGGTGCTGGTGCAATTGCAA452440.133242121791557No Hit
CTCTGAAGCAAAAGCCAAGGCCGGAGCAGGTGC408790.12038733747495932No Hit
CCCCTGGCCCCCCCAAAAGGGGGGTGGTGCTGG389420.11468293490422626No Hit
GTGCTGGTGCAATTGCAAAGGCACAGGAGCACA386520.11382889425088988No Hit
CTCTCTCACTCTCTGAAGCAAAAGCCAAGGCCG386270.11375527005663673No Hit
AGTCTAAGACATGTATCACGAATTGAAAATGAT381550.11236524526913752No Hit
GTGCCAACCCCCCTGGCCCCCCCAAAAGGGGGG376650.11092221106177604No Hit
GAGCACAGGAGCAGCTAAATAAACAAGAAAGCA353720.10416939996487833No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA148150.021.9999871
TTCCGCG500350.019.72287227
GTATCAA571200.017.541271
CGCGGAC76100.017.11864327
CTTCCGC602550.016.36871126
GACCTAG139350.014.58955711
TGACCTA149150.013.83006510
GTGACCT150150.013.764979
GTTGGAG77600.013.344293
CGCCCGC114300.013.22805212
GTGTTGG84700.013.1509551
CCCGCCA114650.013.03456114
CCTAGTA156400.013.03357913
CCGCCCG115300.012.98453311
ACCTAGT158050.012.95731912
GCCCGCC120500.012.81629313
CTAAGAC230500.012.7101493
TGTTGGA86300.012.5626522
GGAACTC76500.012.5468179
AGACATG228050.012.3898256
Produced by FastQC (version 0.11.2)