FastQCFastQC Report
Sat 20 Jan 2018
H5L5MBCX2_l02n01_lib3_dropseq.351000000b9c40.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5L5MBCX2_l02n01_lib3_dropseq.351000000b9c40.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123378996
Sequences flagged as poor quality0
Sequence length27
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTCCGAGCC6193960.502027103543621No Hit
GCTGTCTCTTATACACATCTCCGAGCC4870820.3947851869373293No Hit
ACTGTCTCTTATACACATCTCCGAGCC2962350.2401016458263285No Hit
TCTGTCTCTTATACACATCTCCGAGCC2672700.21662520255878887No Hit
GTCTCTTATACACATCTCCGAGCCCAC2627440.21295683099901383No Hit
ACCTGTCTCTTATACACATCTCCGAGC1876050.1520558653273528No Hit
TGTCTCTTATACACATCTCCGAGCCCA1540470.12485674628119035No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTTTTT20843400.019.98974220
GATTTTT11238250.019.9615919
GGATTTT6467400.019.84681318
GCTTTTT13211450.019.73822819
CTTTTTT26113650.019.67018320
AGTTTTT4077700.019.62937419
GGCTTTT7231700.019.58097618
GTTTTTT49675700.019.45819720
CGTTTTT5515100.019.45701619
TGGTTTT6584550.019.44666518
TATTTTT4386850.019.44541719
TGTTTTT11061750.019.4327519
GGGATTT3629750.019.4200817
GTATTTT2341000.019.37765718
TCTTTTT6335650.019.33428819
GTCTTTT3222150.019.3105518
AGGTTTT3327600.019.30790718
TGATTTT2300250.019.30282418
GAGTTTT2298700.019.30259318
GTGTTTT5835800.019.27118318
Produced by FastQC (version 0.11.2)