FastQCFastQC Report
Sat 20 Jan 2018
H5L5MBCX2_l02n01_lib1212_dropseq.351000000b9c26.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5L5MBCX2_l02n01_lib1212_dropseq.351000000b9c26.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33956229
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTCCGAGCC1347700.3968933063798103No Hit
GCTGTCTCTTATACACATCTCCGAGCC989610.29143695549938714No Hit
ACTGTCTCTTATACACATCTCCGAGCC666060.1961525232969774No Hit
TCTGTCTCTTATACACATCTCCGAGCC646570.19041278111300286No Hit
GTCTCTTATACACATCTCCGAGCCCAC478930.14104334141461938No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA428920.12631555759622187No Hit
ACCTGTCTCTTATACACATCTCCGAGC356340.10494098152065119No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTTTTT6620200.019.99237820
GATTTTT3489700.019.97046919
GGATTTT2000750.019.8129218
CTTTTTT7761600.019.7736120
GCTTTTT3980900.019.76062419
GGCTTTT2228450.019.60198618
TGTTTTT3137350.019.59218819
AGTTTTT1278900.019.55779819
TGATTTT714350.019.54580718
GGGATTT1147850.019.52675217
TATTTTT1473350.019.52572419
CGTTTTT1668250.019.43985219
GGGCTTT1305400.019.3908117
GTGTTTT1582700.019.37677818
GTTTTTT15178400.019.3673720
TGGTTTT1867700.019.33824518
TCTTTTT1892500.019.33671219
GTATTTT731100.019.27464718
TGCTTTT842500.019.23476618
CATTTTT1063000.019.22152919
Produced by FastQC (version 0.11.2)