FastQCFastQC Report
Sat 20 Jan 2018
H5L5MBCX2_l01n02_lib3_dropseq.352000000b9c30.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5L5MBCX2_l01n02_lib3_dropseq.352000000b9c30.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124352776
Sequences flagged as poor quality0
Sequence length33
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACGTACTCTGCGTTGATACCACTGCTTCCGCG5813560.46750544595803795No Hit
ACTTAAATGTGACCTAGTAGATAAAATCATCAT5120350.4117600076736526No Hit
GTCTAAGACATGTATCACGAATTGAAAATGATA5073370.40798204617482764No Hit
CACGTACTCTGCGTTGATACCACTGCTTCCGCG4594470.3694706421350819No Hit
GGTGCATATGGTGCCAACCCCCCTGGCCCCCCC3321320.26708852884796075No Hit
TACGTACTCTGCGTTGATACCACTGCTTCCGCG2802420.22536046963680167No Hit
GTACTCTGCGTTGATACCACTGCTTCCGCGGAC2547430.2048550970828347No Hit
AACGTACTCTGCGTTGATACCACTGCTTCCGCG2536850.20400429178999588No Hit
CCCCAAAAGGGGGGTGGTGCTGGTGCAATTGCA2468620.19851748223135768No Hit
AGTCTAAGACATGTATCACGAATTGAAAATGAT2198710.17681229729845357No Hit
CCCAAAAGGGGGGTGGTGCTGGTGCAATTGCAA2193230.17637161553996994No Hit
GTGTTGGAGGAACTCAAACAAAACAAGCAAAAA2191400.1762244535658778No Hit
GTCTGATACGCCGCCCGCCACGCCCATCGTGTT2112510.16988040540405788No Hit
AGTTATATCAATGCAAACAAAAAAATCTAAAAA2050030.16485599002631032No Hit
GTGCATATGGTGCCAACCCCCCTGGCCCCCCCA1997450.1606276968034875No Hit
CTCTCGAATTGTGTCCGTTGGTAGCCGAAGTTA1889520.1519483569872216No Hit
CATAAAATACACTAAAAAAAGAGAAGACTTACT1842140.14813822893668252No Hit
GTGCTGGTGCAATTGCAAAGGCACAGGAGCACA1812840.14578202902362228No Hit
AAGTTATATCAATGCAAACAAAAAAATCTAAAA1808020.14539442207546696No Hit
CCATAGAGACGTTTAAGGCAAATTCTAAATGAA1773490.14261764449874445No Hit
GTACGTACTCTGCGTTGATACCACTGCTTCCGC1770570.14238282867123128No Hit
CCCCTGGCCCCCCCAAAAGGGGGGTGGTGCTGG1688760.1358039646818982No Hit
GACATGTATCACGAATTGAAAATGATAATGACA1579850.12704581681393265No Hit
GAGCACAGGAGCAGCTAAATAAACAAGAAAGCA1571220.12635182346070023No Hit
CTCGAATTGTGTCCGTTGGTAGCCGAAGTTACG1526150.12272745724631029No Hit
CTCTGAAGCAAAAGCCAAGGCCGGAGCAGGTGC1514450.1217865856086719No Hit
CGTACTCTGCGTTGATACCACTGCTTCCGCGGA1476260.11871548408376506No Hit
GTGCCAACCCCCCTGGCCCCCCCAAAAGGGGGG1458150.11725914345490768No Hit
GGCACAGGAGCACAGGAGCAGCTAAATAAACAA1437450.11559452440370128No Hit
GCACAGGAGCAGCTAAATAAACAAGAAAGCAAA1399570.11254835195637289No Hit
GCTAAATAAACAAGAAAGCAAAATCAACAACCG1320820.10621556208765297No Hit
CTCTCTCACTCTCTGAAGCAAAAGCCAAGGCCG1319180.106083679225625No Hit
GTATAAACCAAAAACAATTGTAACGAGAAAACA1291330.1038440830625285No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA356600.021.2806781
TTCCGCG2338350.018.94896727
CGCGGAC408450.017.71560127
GTATCAA1378800.017.046131
CTTCCGC2830500.015.63420526
GACCTAG989900.015.2277711
TGACCTA1010650.015.02064910
GTGACCT1012800.014.9874299
GGAACTC448350.014.4588919
GTGTTGG496600.014.1557891
CCTAGTA1079250.014.015861513
TTGGAGG484150.013.9088894
ACCTAGT1088650.013.8650812
GTTGGAG495350.013.7635793
CGCCCGC445800.013.6603812
TGTTGGA507400.013.6389312
CCCGCCA456200.013.33121914
GCCCGCC459250.013.27794613
TGTGACC1159200.013.22271258
CTAGTAG1145450.013.123342514
Produced by FastQC (version 0.11.2)