FastQCFastQC Report
Sat 20 Jan 2018
H5L5MBCX2_l01n02_lib1212_dropseq.352000000b9c16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5L5MBCX2_l01n02_lib1212_dropseq.352000000b9c16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34328166
Sequences flagged as poor quality0
Sequence length33
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACGTACTCTGCGTTGATACCACTGCTTCCGCG1268420.36949832973890884No Hit
GTCTAAGACATGTATCACGAATTGAAAATGATA941640.27430536195845706No Hit
CACGTACTCTGCGTTGATACCACTGCTTCCGCG932620.271677782028903No Hit
GGTGCATATGGTGCCAACCCCCCTGGCCCCCCC901290.26255116571039655No Hit
ACTTAAATGTGACCTAGTAGATAAAATCATCAT697810.20327622512662052No Hit
TACGTACTCTGCGTTGATACCACTGCTTCCGCG632370.18421316186830372No Hit
AACGTACTCTGCGTTGATACCACTGCTTCCGCG612580.17844821654614465No Hit
CTCTCGAATTGTGTCCGTTGGTAGCCGAAGTTA611160.17803456205612614No Hit
GTCTGATACGCCGCCCGCCACGCCCATCGTGTT542100.1579169711542411No Hit
GTGCATATGGTGCCAACCCCCCTGGCCCCCCCA524590.1528162034639427No Hit
CTCGAATTGTGTCCGTTGGTAGCCGAAGTTACG502080.146258905879213No Hit
CCCCAAAAGGGGGGTGGTGCTGGTGCAATTGCA500040.14566464168228505No Hit
GTACTCTGCGTTGATACCACTGCTTCCGCGGAC460220.1340648376030342No Hit
CCCAAAAGGGGGGTGGTGCTGGTGCAATTGCAA458830.1336599222923823No Hit
CTCTGAAGCAAAAGCCAAGGCCGGAGCAGGTGC422020.1229369492095791No Hit
CCCCTGGCCCCCCCAAAAGGGGGGTGGTGCTGG397610.11582617026496551No Hit
GTGCTGGTGCAATTGCAAAGGCACAGGAGCACA393340.11458229373512117No Hit
CTCTCTCACTCTCTGAAGCAAAAGCCAAGGCCG393120.11451820641976619No Hit
AGTCTAAGACATGTATCACGAATTGAAAATGAT388730.11323937317245553No Hit
GTGCCAACCCCCCTGGCCCCCCCAAAAGGGGGG382530.11143327610336072No Hit
GAGCACAGGAGCAGCTAAATAAACAAGAAAGCA365600.10650146588081635No Hit
GAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAA350780.10218431127372199No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA147700.021.7636181
TTCCGCG489450.019.73749527
GTATCAA579550.017.5061361
CGCGGAC77100.016.84421327
CTTCCGC591700.016.34496726
GACCTAG141850.014.875092511
TGACCTA150700.014.11799310
GTGACCT152900.013.9766639
GTGTTGG88500.013.7293971
CCTAGTA157250.013.46983613
GTTGGAG86800.013.4694453
ACCTAGT161250.013.08546912
TGTTGGA90300.013.0669792
CCCGCCA114050.013.02051214
CGCCCGC114200.012.9915712
CCGCCCG113750.012.95988811
CTAAGAC236750.012.9445653
GGAACTC86400.012.812399
GCCCGCC119450.012.78222713
AGACATG233600.012.63316
Produced by FastQC (version 0.11.2)