FastQCFastQC Report
Sat 20 Jan 2018
H5L5MBCX2_l01n01_lib3_dropseq.351000000b9c33.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5L5MBCX2_l01n01_lib3_dropseq.351000000b9c33.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124352776
Sequences flagged as poor quality0
Sequence length27
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTCCGAGCC6144600.49412648415665444No Hit
GCTGTCTCTTATACACATCTCCGAGCC4818530.3874887360777535No Hit
ACTGTCTCTTATACACATCTCCGAGCC2930690.2356754786077313No Hit
TCTGTCTCTTATACACATCTCCGAGCC2646860.21285089767517534No Hit
GTCTCTTATACACATCTCCGAGCCCAC2588890.20818916016800462No Hit
ACCTGTCTCTTATACACATCTCCGAGC1858550.14945786172075484No Hit
TGTCTCTTATACACATCTCCGAGCCCA1516280.12193374758276405No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTTTTT21129250.019.98960720
GATTTTT11371900.019.97076419
GGATTTT6518150.019.85829418
GCTTTTT13271100.019.73164219
CTTTTTT26248150.019.68167720
AGTTTTT4131050.019.63174819
GGCTTTT7274900.019.58442318
CGTTTTT5582700.019.47976119
TGGTTTT6654350.019.45783218
GTTTTTT50246700.019.4566620
TATTTTT4453750.019.443719
GGGATTT3663000.019.43864617
TGTTTTT11231900.019.42752819
GTATTTT2358150.019.39650718
TCTTTTT6361350.019.35282919
GAGTTTT2321550.019.32962818
AGGTTTT3350050.019.30757518
GTCTTTT3242900.019.30736418
TGATTTT2348250.019.30568718
GGGCTTT4032300.019.28005817
Produced by FastQC (version 0.11.2)