FastQCFastQC Report
Sat 20 Jan 2018
H5L5MBCX2_l01n01_lib1212_dropseq.351000000b9c19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5L5MBCX2_l01n01_lib1212_dropseq.351000000b9c19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34328166
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTCCGAGCC1336980.3894702676513508No Hit
GCTGTCTCTTATACACATCTCCGAGCC975830.2842651133765783No Hit
ACTGTCTCTTATACACATCTCCGAGCC660440.19239012069564101No Hit
TCTGTCTCTTATACACATCTCCGAGCC639530.18629891267712934No Hit
GTCTCTTATACACATCTCCGAGCCCAC468840.13657589514103374No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA427090.12441387052253242No Hit
ACCTGTCTCTTATACACATCTCCGAGC356050.10371949378245257No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTTTTT6667450.019.95768720
GATTTTT3522450.019.94151319
CTTTTTT7875850.019.79582820
GCTTTTT4037200.019.7778319
GGATTTT2033250.019.7553918
GGCTTTT2247800.019.63683918
TGTTTTT3159100.019.59516119
AGTTTTT1291600.019.57841119
GGGATTT1159700.019.53992317
TGATTTT711350.019.4805718
TATTTTT1475950.019.4798819
CGTTTTT1701300.019.41392119
GTTTTTT15353400.019.38970820
GGGCTTT1308550.019.38899617
TCTTTTT1926400.019.37135519
TGGTTTT1893750.019.3449518
GTGTTTT1599000.019.33218418
AGGTTTT937050.019.24804718
GTATTTT728100.019.24295818
GAGTTTT701450.019.2405918
Produced by FastQC (version 0.11.2)