FastQCFastQC Report
Thu 29 Aug 2019
H5L3MBGXC_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5L3MBGXC_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21755762
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4154651.909677997029017No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTCG19700.022.03008743
CGTTCGA20450.021.39343344
AAGTGCG21300.020.54017639
GTTCGAA22300.019.77386345
AGTGTCG23200.019.3089151
AGTGCGT23200.019.00895740
CGCAAGT23550.018.7257636
GTGCGTT24150.018.5508441
GAACCCG25450.017.4632425
AACCCGG24950.017.3923826
AGGCGTA26100.016.8937058
GTGTCGA27700.016.2985852
ACCCGGG27450.015.8077227
GCGTAGC28250.015.73207910
GGCGTAG28650.015.6344379
TAGCCCC30300.015.13005513
ACAGGCG30850.014.7480626
CGATGAT30350.014.52816456
CCCGGGA32450.014.23534917
GGCCGCA32050.014.19466633
Produced by FastQC (version 0.11.5)