FastQCFastQC Report
Thu 29 Aug 2019
H5L3MBGXC_n01_mb256.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5L3MBGXC_n01_mb256.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29811651
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCGA31450.015.13603244
GCGTTCG33450.014.54488343
AAGTGCG43650.011.22575839
GTTCGAA47450.010.54858745
CGCAAGT48000.010.42696636
GTCCGGT41400.09.8068341
CTCGTAT52150.09.7987281
GTCGCAT25150.09.6024541
CGTATTA11050.09.5020593
CCCGTCT78800.09.4607281
AGTGTCG53600.09.33803651
CTCCGCT97700.09.2068211
AGTGCGT53750.09.05131940
CGCTACT19800.09.0152171
TCGCATT28050.08.9837962
GTGCGTT55200.08.81343941
CCGTAAT48700.08.768031
CTCCGAT72050.08.6954141
TCCGATC44200.08.6310662
AACCCGG57750.08.54528626
Produced by FastQC (version 0.11.5)