FastQCFastQC Report
Thu 29 Aug 2019
H5L3MBGXC_n01_mb255.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5L3MBGXC_n01_mb255.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31912907
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT30050.010.9486911
CGCTATT16150.09.3191181
CTCGTAT53950.09.0178341
GTCCGGT45100.09.0024411
CCCGTCT82800.08.9193051
CCCGAAT53250.08.74200251
CTCCGGT91950.08.716911
CGTATTA12450.08.7148173
TCGTATC39150.08.5823342
CCCCGCT86800.08.5082761
CCCGTTT63800.08.4484861
CGCTACT20600.08.325451
GTCCGCA53850.08.1246221
GTCGCAA16900.08.0771251
CGGGAAT39600.08.043131
CTCAAAT164300.08.0099371
CGCGTAT7908.281131E-87.97488261
GTCGAAT32900.07.87253671
TTAACCG16950.07.84676657
CTCCTAT72100.07.6700941
Produced by FastQC (version 0.11.5)