FastQCFastQC Report
Wed 28 Aug 2019
H5L3MBGXC_n01_mb254.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5L3MBGXC_n01_mb254.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30713439
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT27900.011.0397281
GTCGAAT33300.08.61885451
CCGTAAT46800.08.6006611
CGCTACT20550.08.3457261
CCCGTCT82150.08.2655941
ACGTCCG19700.08.17237232
CTCCGCT100100.08.1470761
TATGCCG18550.08.11356148
GTCCGCT62100.08.0598011
CGGGAAT36550.07.85247141
GTCGCAA16350.07.70663171
GTCCGGT40600.07.6726231
GTCCGTT43600.07.6263551
CGCGTAA6007.223727E-57.58351570
CGGGTAT27900.07.40163561
CCGGGAT58750.07.3874351
CTCGTAT52050.07.32969051
GTCGGTT58650.07.28067641
CGAAAAT22700.07.24689341
GTCGCAT25600.07.24629161
Produced by FastQC (version 0.11.5)