FastQCFastQC Report
Wed 28 Aug 2019
H5L3MBGXC_n01_mb253.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5L3MBGXC_n01_mb253.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23241814
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTACT12600.010.5556081
CCCGTAT27800.010.4496941
GTCCGGT27850.09.4255421
CTCGTAT47000.08.9362151
GTCCGTT41400.08.79231551
GTCGGTT39950.08.5857751
CCGTATT38000.08.5656122
CGGCGCG32700.08.45542724
GTTTATT114900.08.3768911
TCGTATC36550.08.330882
CCCCGCT72450.08.2609111
CCCGCAT42950.08.2305421
CTCCGCT85600.08.2184991
CCCGTCT66250.08.1887221
CTCCGAT53000.08.1887211
AATCCGA42150.08.137382524
GTCGAAT32050.08.0811631
CGTAGTA35250.08.04276766
CTCCGGT81000.08.0370781
GTCGCAT19600.08.0357551
Produced by FastQC (version 0.11.5)