FastQCFastQC Report
Wed 28 Aug 2019
H5L3MBGXC_n01_mb252.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5L3MBGXC_n01_mb252.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25237409
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTAGT10350.013.18841651
CCCGTAT27850.010.5565611
CGTATTA10100.010.0491743
CTCGTAT46600.09.8390641
CGCTACT13900.09.5683531
GTCGCAT17000.09.2647141
CTCCGCT84400.08.9988221
GTCGAAT32450.08.8443831
GTCCGGT29800.08.8087321
CCCGAAT47550.08.7592081
TCGTATC36550.08.3308122
GTCGGAT31550.08.3201331
GTTTATT127250.08.11395551
CTCGCGT8201.8057108E-88.1097631
TCCGCTA13600.07.9777112
GTCGTTT59300.07.96796661
CTTTATT210250.07.9405531
CGGGAAT33750.07.88148741
CGTATCT44650.07.8384883
GTCCGCA46050.07.82845351
Produced by FastQC (version 0.11.5)