FastQCFastQC Report
Wed 28 Aug 2019
H5L3MBGXC_n01_mb251.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5L3MBGXC_n01_mb251.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28775287
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCAT19650.011.7561741
CGTTACG31050.011.15994448
CGTATCG20550.010.90001517
CGCTAGT10400.010.4330911
GTACACT131250.010.1603881
CCCGTAT34350.09.8839281
TATGCCG14750.09.72926948
GTCCGGT32950.09.666531
CGCTACT13150.09.5821141
TCGTTAC43600.09.47290447
GTTACGG62950.09.23015649
CGTATTA8809.094947E-129.1476323
CCGATCG13100.09.0838753
CTCGTAT61800.08.8919251
CTCCGCT97750.08.70111
GACGTAT16700.08.59263517
TCGTATC44850.08.428022
CTCGTTA55450.08.33219246
TTACGGA84950.08.2406750
TGTAGCT226200.08.10815057
Produced by FastQC (version 0.11.5)