FastQCFastQC Report
Wed 28 Aug 2019
H5L3MBGXC_n01_mb250.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5L3MBGXC_n01_mb250.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24974379
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGA1652260.6615820157129834No Hit
GCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCG635630.25451283493375354No Hit
CGCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTC352350.14108458913032432No Hit
CGACGCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCG306760.12282988097521863No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTGC550600.024.7597569
AGTGTCG579500.023.21754351
AAGTGCG582000.023.09884539
GCGTTCG582600.023.02177243
CGTTCGA583100.023.01403444
TCCTGCA595300.023.00641870
GTTCGAA588950.022.83298345
CGCAAGT591150.022.729236
ATGTGTC593650.022.67511465
AGTGCGT591750.022.66502440
GTGCGTT594350.022.56551441
AGGCGTA597750.022.5607998
GTGTCCT603650.022.51998967
ACAGGCG601650.022.4960426
GAACCCG602450.022.23887825
AACCCGG603800.022.20079626
GTGTCGA607450.022.18378452
TAGCCCC614050.022.0182613
CGATGAT612050.022.01006156
TGTGTCC617100.021.91554566
Produced by FastQC (version 0.11.5)