FastQCFastQC Report
Wed 28 Aug 2019
H5L3MBGXC_n01_mb249.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5L3MBGXC_n01_mb249.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29973090
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGA1583450.5282905432839924No Hit
GCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCG611440.2039963180306068No Hit
CGCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTC342190.11416574000211524No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCGA548200.023.87927444
GCGTTCG553550.023.64844543
AAGTGCG556700.023.57648339
AGTGTCG555550.023.52523251
CGCAAGT560900.023.33707636
AGGCGTA564050.023.3317438
AGTGCGT562750.023.31695440
GTTCGAA564600.023.21068245
GTGCGTT566900.023.15830241
TGTTACG44100.023.0947483
GCCGCAA570700.022.94856334
AACCCGG573200.022.82393626
GAACCCG579750.022.70484425
CCGCAAG577950.022.67889435
GTCCTGC583150.022.67570769
GTGTCGA580550.022.5182452
ACAGGCG589650.022.3838166
GCGTAGC589250.022.37562410
ACCCGGG592250.022.1134327
CGATGAT596100.022.04704556
Produced by FastQC (version 0.11.5)