FastQCFastQC Report
Wed 28 Aug 2019
H5L3MBGXC_n01_mb248.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5L3MBGXC_n01_mb248.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23947065
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT21000.09.5001021
GTCGCGA6151.2768214E-79.1057881
GTCCGCA41400.08.8769061
CCCGTCT65550.08.7567681
GTCCGTT34400.08.6483491
GTCGGTT40000.08.4875911
GTCCGGT32550.08.3871871
CCCGAAT42150.08.3037671
GTCCGCT44800.08.281341
GTCGCAT19900.08.266421
CCGGCAT45150.08.2171431
CGCTACT16200.08.2099651
CGGCAAT35950.07.98339561
CGGGAAT30850.07.8282851
CCGGGAT43300.07.75989871
CCGTAAT37450.07.7570931
CTCGAAT68800.07.6308961
CGCTAAT10605.14774E-107.5944211
GTCCGAT44300.07.58473161
CCCGCAT38450.07.55534741
Produced by FastQC (version 0.11.5)