FastQCFastQC Report
Wed 28 Aug 2019
H5L3MBGXC_n01_mb247.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5L3MBGXC_n01_mb247.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27958936
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG329470.11784067891567834No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT54550.011.677481
CTCATAT93500.010.1444891
CCCGTAT30400.010.1316811
CTCCGCT97100.09.8765171
CTCCGTT70600.09.32020951
CTCCGAT67250.09.10791
CGCTAGT10150.08.965611
CTCGCGT8202.1282176E-108.9635061
CCCCGCT79500.08.8051211
CTCCGGT84250.08.6825821
CTTTATT233600.08.6452081
ACGTATA17050.08.62143418
CCCGAAT51200.08.6133691
CCCGTTT58750.08.5788111
CGTATTA11350.08.3258833
CGGCGCG30150.08.24191224
CTCCTAT65600.08.2165471
CTCTAAT58600.08.1826771
CCCGTCT76000.08.0592931
CTCGAAT93450.08.0525171
Produced by FastQC (version 0.11.5)