FastQCFastQC Report
Wed 28 Aug 2019
H5L3MBGXC_n01_mb246.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5L3MBGXC_n01_mb246.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28208212
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG16850.013.50199746
ACGTATA17450.012.83636918
GACGTAT18300.011.85765917
CGTATTA8300.011.3853493
CGCGTAA5754.114554E-910.34803670
CCCGTAT31500.09.7779591
GTCCGGT36100.09.59851551
CTCGTAT57650.09.5318341
GACTACG24750.09.47463735
GTCCGCA55100.08.9566091
CTCCGCT100400.08.8547461
TCGTATC40550.08.8037652
CGTATAG24800.08.74975919
CGCTATA7807.399649E-98.52581
GTCGCAT20950.08.5204441
CGTTACG18950.08.31167748
GTTTATT138750.08.2740281
GCCGTTT43650.08.01847651
TCCGCTA12400.07.90306432
CTTTATT232850.07.87646151
Produced by FastQC (version 0.11.5)