FastQCFastQC Report
Wed 28 Aug 2019
H5L3MBGXC_n01_mb245.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5L3MBGXC_n01_mb245.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30524960
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTCG115200.019.44531843
CGTTCGA115300.019.3677744
AAGTGCG116550.019.27960639
GTTCGAA122350.018.50929845
AGTGTCG122350.018.30865351
AGTGCGT128550.017.47987740
CGCAAGT130950.017.18587536
GTGCGTT132000.017.07579641
CGTATTA10050.017.0642973
GAACCCG139750.016.12841625
AACCCGG141300.016.07536726
AGGCGTA144550.015.6419898
GTGTCGA153400.014.76250552
TAGCCCC157450.014.51544413
GCCGCAA159800.014.36770934
ACAGGCG160400.014.358176
ACCCGGG163300.014.188292527
CCGCAAG161400.014.1170435
TGCGTTC167750.013.72922142
GCGTAGC166000.013.70516410
Produced by FastQC (version 0.11.5)