FastQCFastQC Report
Wed 28 Aug 2019
H5L3MBGXC_n01_mb244.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5L3MBGXC_n01_mb244.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36613182
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGA445570.12169660642989183No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG29650.029.27643246
GCGTTCG185150.020.20884943
CGTTCGA187150.019.97426444
AAGTGCG188900.019.807139
AGTGTCG195000.019.17009451
GTTCGAA199050.018.86817445
AGTGCGT205100.018.37916640
CGCAAGT207400.018.20862436
GTGCGTT209250.017.89725141
AACCCGG216650.017.56019626
GAACCCG218550.017.5035725
TATCTCG48750.017.37464339
AGGCGTA222050.017.102198
ACAGGCG239400.016.3160786
GCCGCAA236700.016.10241934
GTGTCGA234000.016.0647652
ACCCGGG239550.015.99839727
TAGCCCC238250.015.95363213
CCGCAAG241600.015.66005235
CGTATCG43000.015.54602117
Produced by FastQC (version 0.11.5)