FastQCFastQC Report
Fri 22 Nov 2024
H5L3LBGYW_n01_mid_L4_males.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5L3LBGYW_n01_mid_L4_males.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20241520
Sequences flagged as poor quality0
Sequence length15
%GC36

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGTATGCAGCGCG11997035.926941257375928No Hit
CAAAAAAAAAAAAAA10437995.156722420055411No Hit
AAAAAAAAAAAAAAA10333685.105189728834594No Hit
CCAAAAAAAAAAAAA4277192.1130774763950533No Hit
TAAAAAAAAAAAAAA1957000.9668246258186144No Hit
ACAAAAAAAAAAAAA1594560.7877669265944455No Hit
GAAAAAAAAAAAAAA1270580.6277097767361345No Hit
CTAAAAAAAAAAAAA1163020.5745714748694762No Hit
CCAGTATGCAGCGCG1072860.5300293653836273No Hit
CTAGTATGCAGCGCG978500.4834123129093072No Hit
ATAAAAAAAAAAAAA957030.47280540196586024No Hit
AAGTATGCAGCGCGC949270.4689716977776373No Hit
CCCAAAAAAAAAAAA895840.4425754587600141No Hit
CAAGTATGCCGCGCG893620.4414787031803936No Hit
GCAAAAAAAAAAAAA644280.31829625443148535No Hit
AAAGTATGCAGCGCG623690.30812409344752767No Hit
CAAGTATGCAGCGCC602910.2978580659950439No Hit
ACCAAAAAAAAAAAA581980.28751793343582893No Hit
AATAAAAAAAAAAAA534500.26406119698520664No Hit
GAAGTATGCAGCGCG529480.26158114607993865No Hit
TCAAAAAAAAAAAAA526990.26035100130820216No Hit
TCCAAAAAAAAAAAA467930.23117335061793778No Hit
CACAAAAAAAAAAAA453130.2238616467538011No Hit
AACAAAAAAAAAAAA443480.21909421822076605No Hit
CATAAAAAAAAAAAA363390.17952703156679933No Hit
CAAGGATGCAGCGCG341030.16848043032341445No Hit
TTAAAAAAAAAAAAA340630.1682828167054648No Hit
CATGTATGCAGCGCG325840.16097605318177688No Hit
GCCAAAAAAAAAAAA318330.15726585750477237No Hit
GTAAAAAAAAAAAAA318170.1571868120575925No Hit
CAAATAAAAAAAAAA314010.15513163043091627No Hit
AAAATAAAAAAAAAA291440.1439812820381078No Hit
GTAGTATGCAGCGCG279400.13803311213782365No Hit
AAAACAAAAAAAAAA275580.13614590208640456No Hit
CCTAAAAAAAAAAAA266950.1318823882791411No Hit
CAAGTCTGCAGCGCG264910.1308745588275979No Hit
CAAGTATGCAGCGGG260400.12864646528521573No Hit
CACGTATGCAGCGCG239450.11829645204510333No Hit
CAAGTATGCAGCCCG227190.11223959465494686No Hit
CAAACAAAAAAAAAA222710.11002632213391089No Hit
AAAAAAAAAAATAAA221980.1096656772811528No Hit
CGAAAAAAAAAAAAA219140.10826262059371035No Hit
CCAAGTATGCAGCGC218990.10818851548697925No Hit
ATCAAAAAAAAAAAA213220.10533793904805569No Hit
ATAGTATGCAGCGCG203900.10073354174982906No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)