FastQCFastQC Report
Fri 22 Nov 2024
H5L3LBGYW_n01_mid_L4_males_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5L3LBGYW_n01_mid_L4_males_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12874158
Sequences flagged as poor quality0
Sequence length15
%GC34

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAAAAAAAAAAAA8307886.45314435320741No Hit
AAAAAAAAAAAAAAA6191594.80931646170569No Hit
CAAGTATGCAGCGCG5043783.9177552427117948No Hit
CCAAAAAAAAAAAAA3927673.0508169932355966No Hit
TAAAAAAAAAAAAAA1167310.9067078406214993No Hit
ACAAAAAAAAAAAAA1125530.8742552328470723No Hit
GAAAAAAAAAAAAAA794450.6170889001051564No Hit
CTAAAAAAAAAAAAA760170.5904619160336544No Hit
CCCAAAAAAAAAAAA691220.5369050154580983No Hit
AAGTATGCAGCGCGC640730.497686916690008No Hit
ATAAAAAAAAAAAAA580930.4512372770320202No Hit
CCAGTATGCAGCGCG553500.42993102927585636No Hit
CTAGTATGCAGCGCG470480.3654452586336132No Hit
CACAAAAAAAAAAAA459710.357079662996213No Hit
CAAGTATGCCGCGCG393050.3053015195246167No Hit
GCAAAAAAAAAAAAA388850.30203917025097876No Hit
AAAGTATGCAGCGCG377230.29301333726058043No Hit
TCAAAAAAAAAAAAA332660.2583935974686655No Hit
ACCAAAAAAAAAAAA330440.2566692128525998No Hit
AATAAAAAAAAAAAA301630.23429105033509764No Hit
CATAAAAAAAAAAAA274990.21359843494230846No Hit
TCCAAAAAAAAAAAA266600.20708150389330315No Hit
AACAAAAAAAAAAAA259380.20147337014195413No Hit
GAAGTATGCAGCGCG253000.1965177062453327No Hit
CAAGTATGCAGCGCC222330.17269478904950522No Hit
TTAAAAAAAAAAAAA199110.15465865806524978No Hit
CAAATAAAAAAAAAA196390.1525458985356557No Hit
GTAAAAAAAAAAAAA195340.1517303112172462No Hit
CCTAAAAAAAAAAAA191380.14865438190210187No Hit
TAAGTATGCAGCGCG185420.14402495293284423No Hit
CGAAAAAAAAAAAAA170820.13268440545781712No Hit
ACAGTATGCAGCGCG164420.12771320656465454No Hit
ATAGTATGCAGCGCG162930.12655584932234015No Hit
CAAACAAAAAAAAAA160400.12459067225988683No Hit
AAAATAAAAAAAAAA160330.12453629977199285No Hit
AAAACAAAAAAAAAA156910.1218798153634591No Hit
GCCAAAAAAAAAAAA156230.12135162548106057No Hit
CAAGGATGCAGCGCG148170.11509102187498399No Hit
CATGTATGCAGCGCG144330.11210830253908645No Hit
GTAGTATGCAGCGCG141640.11001884550430406No Hit
CCAAGTATGCAGCGC135020.10487676164918902No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)