FastQCFastQC Report
Fri 22 Nov 2024
H5L3LBGYW_n01_mid_L4_herms.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5L3LBGYW_n01_mid_L4_herms.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29347181
Sequences flagged as poor quality0
Sequence length15
%GC36

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGTATGCAGCGCG24209428.249317029802624No Hit
AAAAAAAAAAAAAAA17365805.917365623635197No Hit
CAAAAAAAAAAAAAA16583745.65088006238146No Hit
CCAAAAAAAAAAAAA5780681.9697564818917361No Hit
TAAAAAAAAAAAAAA3101531.0568408597745726No Hit
ACAAAAAAAAAAAAA2428410.8274764107666763No Hit
GAAAAAAAAAAAAAA1973890.6725995249765215No Hit
CAAGTATGCCGCGCG1781240.6069543783438689No Hit
CTAAAAAAAAAAAAA1740320.5930109607461105No Hit
CCAGTATGCAGCGCG1635180.557184691776699No Hit
CTAGTATGCAGCGCG1503740.5123967443414753No Hit
ATAAAAAAAAAAAAA1460530.49767301329555297No Hit
AAGTATGCAGCGCGC1336060.4552600810278848No Hit
CCCAAAAAAAAAAAA1310850.4466698181334691No Hit
CAAGTATGCAGCGCC1244980.42422473218126133No Hit
ACCAAAAAAAAAAAA1022540.34842869575786517No Hit
AAAGTATGCAGCGCG934070.3182826997932101No Hit
TCAAAAAAAAAAAAA871870.2970881598474484No Hit
GAAGTATGCAGCGCG869960.2964373307269274No Hit
GCAAAAAAAAAAAAA850960.28996311434478156No Hit
AATAAAAAAAAAAAA804410.27410128420852414No Hit
TCCAAAAAAAAAAAA748510.2550534581157897No Hit
CACAAAAAAAAAAAA687490.2342610010821823No Hit
AACAAAAAAAAAAAA655020.22319690603332565No Hit
CAAGGATGCAGCGCG624360.21274956528192607No Hit
CATAAAAAAAAAAAA529230.1803341861012136No Hit
CATGTATGCAGCGCG526250.17931875637390862No Hit
CAAGTATGCAGCGGG494280.1684250354403716No Hit
AAAATAAAAAAAAAA488520.16646232563188948No Hit
CAAGTCTGCAGCGCG484670.16515044494392833No Hit
TTAAAAAAAAAAAAA473890.16147717901763717No Hit
GCCAAAAAAAAAAAA466530.15896927204013223No Hit
CAAATAAAAAAAAAA464470.15826733068501536No Hit
CAAGTATGCAGCCCG443170.15100939337239921No Hit
CACGTATGCAGCGCG425530.14499859458392272No Hit
CGAAAAAAAAAAAAA421360.14357767446215702No Hit
AAAACAAAAAAAAAA421040.14346863502835247No Hit
CCAAGTATGCAGCGC405170.13806096060810746No Hit
GTAAAAAAAAAAAAA404980.137996218444286No Hit
GTAGTATGCAGCGCG402790.13724997981918605No Hit
TAAGTATGCAGCGCG389060.13257150661250905No Hit
CAAGTATGCAGCGCT367370.1251806774899436No Hit
ATCAAAAAAAAAAAA351820.11988204250350315No Hit
CCTAAAAAAAAAAAA350120.1193027705114164No Hit
CAAACAAAAAAAAAA317110.10805467141801456No Hit
CAAGTATGCAGCCCC315060.10735613754520408No Hit
CAATTATGCAGCGCG300920.102537957563965No Hit
CAAGTATGCAGCGAG296480.1010250354199267No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)