FastQCFastQC Report
Fri 22 Nov 2024
H5L3LBGYW_n01_late_L4_males.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5L3LBGYW_n01_late_L4_males.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20721414
Sequences flagged as poor quality0
Sequence length15
%GC38

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGTATGCAGCGCG222458210.735666977166712No Hit
CAAAAAAAAAAAAAA11329385.467474372163984No Hit
AAAAAAAAAAAAAAA9651064.657529645418985No Hit
CCAAAAAAAAAAAAA4676182.25668962552459No Hit
TAAAAAAAAAAAAAA1800480.8688982325240933No Hit
CAAGTATGCCGCGCG1682330.8118799228662676No Hit
ACAAAAAAAAAAAAA1485160.7167271499908259No Hit
AAGTATGCAGCGCGC1467820.7083589951921235No Hit
CCAGTATGCAGCGCG1434480.6922693596103046No Hit
CTAGTATGCAGCGCG1377570.6648050176498572No Hit
GAAAAAAAAAAAAAA1200130.579173795765096No Hit
CTAAAAAAAAAAAAA1120520.5407546029436022No Hit
CCCAAAAAAAAAAAA1094740.5283133670318059No Hit
CAAGTATGCAGCGCC1015990.4903092038024046No Hit
ATAAAAAAAAAAAAA781490.37714125107485424No Hit
AAAGTATGCAGCGCG780470.37664900667493056No Hit
GAAGTATGCAGCGCG705040.340247050708026No Hit
ACCAAAAAAAAAAAA637930.307860264748342No Hit
CAAGGATGCAGCGCG548250.26458136495897433No Hit
GCAAAAAAAAAAAAA524690.25321148450583536No Hit
CACAAAAAAAAAAAA498520.24058203749995052No Hit
TCAAAAAAAAAAAAA490170.23655238971626164No Hit
CATGTATGCAGCGCG475190.22932315333306888No Hit
TCCAAAAAAAAAAAA475090.2292748940781744No Hit
AATAAAAAAAAAAAA423640.2044455074349656No Hit
CAAGTCTGCAGCGCG414460.20001530783565255No Hit
CCAAGTATGCAGCGC393440.1898712124568333No Hit
CAAGTATGCAGCGGG390840.1886164718295769No Hit
AACAAAAAAAAAAAA380230.1834961648852728No Hit
CACGTATGCAGCGCG379120.18296048715594407No Hit
CAAGTATGCAGCCCG364290.17580363965509302No Hit
GTAGTATGCAGCGCG342780.1654230739272909No Hit
CATAAAAAAAAAAAA335910.16210766311604025No Hit
GCCAAAAAAAAAAAA306750.14803526438881054No Hit
CAAGTATGCAGCGCT306440.14788566069863765No Hit
CAAATAAAAAAAAAA306110.1477264051574859No Hit
AAAATAAAAAAAAAA262520.126690195948983No Hit
CGAAAAAAAAAAAAA261500.12619795154905936No Hit
TTAAAAAAAAAAAAA259590.12527619978057483No Hit
TAAGTATGCAGCGCG256560.12381394435727214No Hit
CCTAAAAAAAAAAAA250030.12066261501266275No Hit
CAAGTATTCAGCGCG248340.11984703360494607No Hit
CAAGTATGCAGCCCC245900.11866950778552082No Hit
GTAAAAAAAAAAAAA238220.11496319700962492No Hit
ATAGTATGCAGCGCG235910.11384840822156249No Hit
CAATTATGCAGCGCG231380.11166226397484263No Hit
CAACTATGCAGCGCG230300.11114106402198229No Hit
CAAGTAAGCAGCGCG230250.11111693439453504No Hit
CAAGTATGCAGCTCG224850.10851093463023324No Hit
AAAACAAAAAAAAAA214810.10366570543882767No Hit
CAAGTATGCAGCGAG214620.10357401285452816No Hit
ACAGTATGCAGCGCG213540.10305281290166782No Hit
CAAGTATGCAGCGCA209120.1009197538353319No Hit
CAAGTATGCAGCCGC208730.10073154274124343No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)