FastQCFastQC Report
Fri 22 Nov 2024
H5L3LBGYW_n01_late_L3_early_L4_males.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5L3LBGYW_n01_late_L3_early_L4_males.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1060232
Sequences flagged as poor quality0
Sequence length15
%GC41

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAA190771.7993231670049574No Hit
CAAGTATGCAGCGCG172381.6258705641784061No Hit
CAAAAAAAAAAAAAA136151.2841529023836293No Hit
CCAAAAAAAAAAAAA52030.4907416489975779No Hit
TAAAAAAAAAAAAAA33180.31295037312588186No Hit
ACAAAAAAAAAAAAA30220.2850319552701673No Hit
ATAAAAAAAAAAAAA21160.19957896007666248No Hit
GAAAAAAAAAAAAAA18540.17486738751518535No Hit
CAAGTATGCCGCGCG17900.16883097284367948No Hit
CTAGTATGCAGCGCG17010.16043658369111666No Hit
CTAAAAAAAAAAAAA16430.15496608289506447No Hit
CCAGTATGCAGCGCG16300.15373993616491485No Hit
CCCAAAAAAAAAAAA14460.13638524398433552No Hit
TGGAATTCTCGGGTG13630.12855676870722635Illumina Small RNA Adapter 2 (100% over 15bp)
AATAAAAAAAAAAAA12610.11893623282451388No Hit
ACCAAAAAAAAAAAA12550.1183703189490602No Hit
AACAAAAAAAAAAAA11030.10403383410423378No Hit
CAAGTATGCAGCGCC10940.10318496329105328No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)