FastQCFastQC Report
Fri 22 Nov 2024
H5L3LBGYW_n01_late_L3_early_L4_herms.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5L3LBGYW_n01_late_L3_early_L4_herms.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23011014
Sequences flagged as poor quality0
Sequence length15
%GC42

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGTATGCAGCGCG10244884.452163646504235No Hit
AAAAAAAAAAAAAAA4154091.8052616021180117No Hit
CAAAAAAAAAAAAAA3770071.6383763010182864No Hit
CCAAAAAAAAAAAAA1471330.6394025052524848No Hit
CAAGTATGCCGCGCG822140.3572810828762262No Hit
AAGTATGCAGCGCGC820190.3564336625930522No Hit
TAAAAAAAAAAAAAA777160.33773392167768007No Hit
CCAGTATGCAGCGCG726860.31587482411683376No Hit
CTAGTATGCAGCGCG708110.307726552163238No Hit
ACAAAAAAAAAAAAA640640.27840581036541895No Hit
GAAAAAAAAAAAAAA467160.20301582537822974No Hit
CTAAAAAAAAAAAAA443690.1928163617648488No Hit
CAAGTATGCAGCGCC443290.19264253196317208No Hit
ATAAAAAAAAAAAAA415470.18055266925655689No Hit
AAAGTATGCAGCGCG410740.17849713185172977No Hit
CCCAAAAAAAAAAAA386020.16775445010810908No Hit
GAAGTATGCAGCGCG359510.15623388000198513No Hit
CCAAGTATGCAGCGC338210.14697744306270033No Hit
ACCAAAAAAAAAAAA271790.1181130044942826No Hit
CAAGGATGCAGCGCG266680.11589232877786262No Hit
CATGTATGCAGCGCG247600.10760064723788357No Hit
AATAAAAAAAAAAAA243630.10587538645624223No Hit
TCAAAAAAAAAAAAA234640.10196856166355815No Hit
GCAAAAAAAAAAAAA230320.10009119980544968No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)