FastQCFastQC Report
Fri 22 Nov 2024
H5L3LBGYW_n01_early_L4_mid_L4_males.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5L3LBGYW_n01_early_L4_mid_L4_males.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7075331
Sequences flagged as poor quality0
Sequence length15
%GC41

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGTATGCAGCGCG2293773.24192606678048No Hit
AAAAAAAAAAAAAAA1691992.391393420321961No Hit
CAAAAAAAAAAAAAA1579892.2329556030664857No Hit
CCAAAAAAAAAAAAA665150.9400973608160523No Hit
TAAAAAAAAAAAAAA334200.47234539274558324No Hit
ACAAAAAAAAAAAAA275710.38967788220791366No Hit
GAAAAAAAAAAAAAA220510.3116603308028981No Hit
CCCAAAAAAAAAAAA214870.303688972289777No Hit
CTAAAAAAAAAAAAA196270.27740044953373916No Hit
CAAGTATGCCGCGCG191610.2708141852303447No Hit
CCAGTATGCAGCGCG179300.25341570592245083No Hit
ATAAAAAAAAAAAAA173480.24518994235040026No Hit
CTAGTATGCAGCGCG170730.24130319839453448No Hit
AAGTATGCAGCGCGC167880.23727511829481898No Hit
CAAGTATGCAGCGCC140120.19804020476215176No Hit
ACCAAAAAAAAAAAA129620.18319990965793687No Hit
GCAAAAAAAAAAAAA113000.15970984255012238No Hit
TCAAAAAAAAAAAAA105840.14959017465048632No Hit
AAAGTATGCAGCGCG104580.14780933923798054No Hit
TCCAAAAAAAAAAAA98780.1396118428946999No Hit
GAAGTATGCAGCGCG96830.13685578808963142No Hit
AATAAAAAAAAAAAA94740.13390186268317342No Hit
AACAAAAAAAAAAAA78300.11066620063428835No Hit
CACAAAAAAAAAAAA71150.10056066634903725No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)