FastQCFastQC Report
Fri 22 Nov 2024
H5L3LBGYW_n01_early_L4_mid_L4_herms.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5L3LBGYW_n01_early_L4_mid_L4_herms.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9877976
Sequences flagged as poor quality0
Sequence length15
%GC40

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAA2361522.390692182285116No Hit
CAAAAAAAAAAAAAA2119432.1456116111235746No Hit
CAAGTATGCAGCGCG2013092.037957978436068No Hit
CCAAAAAAAAAAAAA906880.9180828137262127No Hit
TAAAAAAAAAAAAAA434680.44004966199553436No Hit
ACAAAAAAAAAAAAA363950.3684459245497256No Hit
CCCAAAAAAAAAAAA281560.28503814951564976No Hit
GAAAAAAAAAAAAAA272190.27555240061324304No Hit
CTAAAAAAAAAAAAA251230.25433347884222435No Hit
ATAAAAAAAAAAAAA225770.22855896795051941No Hit
CAAGTATGCCGCGCG181960.18420777697779384No Hit
ACCAAAAAAAAAAAA174550.17670624022573045No Hit
CCAGTATGCAGCGCG161300.16329256114815424No Hit
CTAGTATGCAGCGCG151060.15292606501574815No Hit
AAGTATGCAGCGCGC143500.14527267529299526No Hit
TCCAAAAAAAAAAAA132130.13376222011472794No Hit
TCAAAAAAAAAAAAA131100.13271949638265976No Hit
GCAAAAAAAAAAAAA128180.12976342521990336No Hit
AATAAAAAAAAAAAA126410.12797156016576675No Hit
CAAGTATGCAGCGCC123490.12501548900301032No Hit
AACAAAAAAAAAAAA107820.10915191533164284No Hit
TGGAATTCTCGGGTG105040.10633757360819665Illumina Small RNA Adapter 2 (100% over 15bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)