FastQCFastQC Report
Fri 22 Nov 2024
H5L3LBGYW_n01_WT_26_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5L3LBGYW_n01_WT_26_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6195078
Sequences flagged as poor quality0
Sequence length15
%GC40

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGTATGCAGCGCG2275563.673174090786266No Hit
AAAAAAAAAAAAAAA1817582.9339097909663123No Hit
CAAAAAAAAAAAAAA1588362.5639063785798983No Hit
CCAAAAAAAAAAAAA750051.2107192193544618No Hit
TAAAAAAAAAAAAAA387260.6251091592389959No Hit
ACAAAAAAAAAAAAA309200.49910590310565905No Hit
CCCAAAAAAAAAAAA234690.37883300258689234No Hit
AAGTATGCAGCGCGC203070.3277924830002141No Hit
GAAAAAAAAAAAAAA197390.31862391401690177No Hit
CAAGTATGCCGCGCG197240.31838178631487773No Hit
CTAAAAAAAAAAAAA172250.27804331115766423No Hit
ATAAAAAAAAAAAAA164220.26508140817597453No Hit
CCAGTATGCAGCGCG160020.2583018325193No Hit
CTAGTATGCAGCGCG157710.254573065908129No Hit
CAAGTATGCAGCGCC146210.23600994208628204No Hit
TGGAATTCTCGGGTG127990.2065994972137558Illumina Small RNA Adapter 2 (100% over 15bp)
ACCAAAAAAAAAAAA118620.19147458676065093No Hit
CCAAGTATGCAGCGC111570.18009458476551868No Hit
TCAAAAAAAAAAAAA100630.1624354043645617No Hit
CACAAAAAAAAAAAA97560.1574798573964686No Hit
CAAGTATGCAGCGGG94870.1531377006068366No Hit
AATAAAAAAAAAAAA94460.15247588488797073No Hit
GCAAAAAAAAAAAAA90410.1459384369333203No Hit
TCCAAAAAAAAAAAA87840.14178998230530754No Hit
AACAAAAAAAAAAAA84580.13652774024798398No Hit
CAAGTATGCAGCGCT80670.130216278148556No Hit
GAAGTATGCAGCGCG77460.12503474532524045No Hit
AAAGTATGCAGCGCG77370.124889468704026No Hit
CAAGTATGCAGCGCA71830.11594688557593626No Hit
CAAGGATGCAGCGCG65120.1051157063720586No Hit
AAAATAAAAAAAAAA62960.10162906746291168No Hit
TTAAAAAAAAAAAAA61960.10001488278275107No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)