FastQCFastQC Report
Fri 22 Nov 2024
H5L3LBGYW_n01_WT_25_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5L3LBGYW_n01_WT_25_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1202934
Sequences flagged as poor quality0
Sequence length15
%GC36

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAA465833.87244853001079No Hit
CAAAAAAAAAAAAAA355132.952198541233351No Hit
CAAGTATGCAGCGCG310412.580440822189746No Hit
CCAAAAAAAAAAAAA137441.1425398234649615No Hit
ACAAAAAAAAAAAAA73020.6070158462559043No Hit
TAAAAAAAAAAAAAA65830.5472453185295286No Hit
TGCAACTCCAGTCTT53600.44557723033848906No Hit
ATAAAAAAAAAAAAA46410.38580670261211336No Hit
CTAAAAAAAAAAAAA43190.35903881675968924No Hit
GAAAAAAAAAAAAAA36410.30267662232508186No Hit
CCCAAAAAAAAAAAA33820.28114593153074063No Hit
ACCAAAAAAAAAAAA27240.22644633870187392No Hit
CCAGTATGCAGCGCG25910.21539003802369872No Hit
CAAGTATGCCGCGCG24980.2076589405570048No Hit
AATAAAAAAAAAAAA24940.20732642023585665No Hit
TGCACCTCCAGTCTT24620.20466625766667165No Hit
CTAGTATGCAGCGCG24210.20125792437490336No Hit
AACAAAAAAAAAAAA21250.17665142060994202No Hit
TCAAAAAAAAAAAAA19200.15960975415110057No Hit
GCAAAAAAAAAAAAA19010.15803028262564695No Hit
CAAGTATGCAGCGCC16180.13450446990441703No Hit
CACAAAAAAAAAAAA16030.13325751870011157No Hit
TCCAAAAAAAAAAAA15870.13192743741551907No Hit
TGGAATTCTCGGGTG15210.12644085211657496Illumina Small RNA Adapter 2 (100% over 15bp)
AAAGTATGCAGCGCG15130.12577581147427871No Hit
CATAAAAAAAAAAAA13970.11613272216098307No Hit
AAAATAAAAAAAAAA13410.1114774376649093No Hit
AAAACAAAAAAAAAA13020.10823536453371506No Hit
TTAAAAAAAAAAAAA12900.1072378035702707No Hit
AAGTATGCAGCGCGC12250.10183434835161363No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)