FastQCFastQC Report
Thu 23 Feb 2023
H5L25AFX5_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5L25AFX5_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences152733691
Sequences flagged as poor quality0
Sequence length16
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGGAACCTTTGACTA98984076.480827468511842No Hit
TGCCAGAACATGATCG89264475.844451830866839No Hit
CCAAGGTACTAGGACC73319704.8004929050002465No Hit
AAAGGTTCTAGGATCG58121773.8054321623118503No Hit
TCCTCCTACCTAGTAA55771703.6515649975354814No Hit
TCCTAGTACTAGCGGT52092603.4106816681330643No Hit
TGTGAGGTGGTTCACG51731743.3870549229377294No Hit
CTTTGTTTGCACAGGA40658722.6620662234896164No Hit
GTGGTCGCCGTATCAT36596392.3960915080615712Illumina Single End PCR Primer 1 (100% over 16bp)
CGTATTTGAGACTCAG26094011.7084645718409306No Hit
TTTGCTCCTTTGCTCT24417841.5987199576025437No Hit
TTGCTTAGATTATGTC23874581.5631508571347235No Hit
CACCAACCTTCGCTTT18303481.1983917811558682No Hit
GGATATTGATGTAACG17238541.128666497033716No Hit
CATGGTCCTCAATTAC17076161.1180349200098882No Hit
CTGGCAGGATCAGCCT16048131.0507262605210006No Hit
CTGACTATGCCTATTT15209580.9958235082526748No Hit
ACACTTGCTCCCTTTA13507570.8843870603506857No Hit
AATTGTGGAACCGAAC12746170.8345355839007387No Hit
CAGGTTCCTAGTCTCA11092980.7262955492904313No Hit
CGTATTTGATAAAGGA9396000.6151884327865814No Hit
GTCCTCCTGTTGCAGT7779620.5093584754656391No Hit
CGAGGTTACTAATCGA7460870.48848881678633693No Hit
ATATGCGACAAAGGTA6198690.40584955155703006No Hit
TAGCAAGCTATGTGGG6146200.4024128507442408No Hit
GTCTGGGACCAGTAGC5930870.38831445512568674No Hit
CATGTTTGATGGCTTC5581530.3654419639475615No Hit
TAAGCACGACTAAGGT5348740.3502004020841741No Hit
TCCCTGTTGGTTATGT5214160.3413889866643765No Hit
CCATTATGAGCAAGAA5187610.33965066685908873No Hit
GCGGTCAACCAAGCGT4409020.28867370199283665No Hit
GCGTGACACGTAATCG4383670.28701395031434157No Hit
GCGGATACTACGGATG3951990.25875037616945956No Hit
GTTGCATCTAAGTCCT3874180.253655887881345No Hit
GCCTCAATGCGCCACA3721700.24367249790355686No Hit
CTGCTCCCTCCACCCT3669390.24024758230978654No Hit
GTAAACTGATGTTGCC3636410.23808826829176807No Hit
TTGCCAGCTCACAGAA3442500.225392313736463No Hit
TGAGCTTTGGATGTAG3098080.20284195187818777No Hit
TGAACGGTGCATGTCA2851710.18671126071326333No Hit
TCCCTCATGGATTGCC2734660.17904759467902862No Hit
TTTCGTCCTACAACAA2679460.17543346084656594No Hit
TTCTAAGCTTAAGGGT2625880.17192539398527337No Hit
GGCCAGTACTTCATGC2442750.15993524310232246No Hit
TCGTCCTTGCTTGAAC2424640.15874951912214313No Hit
GCTACAAACTTAAAGC2355760.15423970864424405No Hit
GGTTACAACCGGCAAT2333020.152750842641523No Hit
TCCCATAACTAAGGAG2291980.1500638127052138No Hit
TTATCACGATTTGAGG2283500.14950859794254562No Hit
CTATCGGCTTCCAGCA2247530.14715351834193546No Hit
TCCCTTTGATTGAGTG2116870.13859875880299388No Hit
TTGGACCCTGGTCTAT1995870.1306764726847333No Hit
CCACAAAGAGATTATG1990340.13031440456709711No Hit
AACCATGACCTGGTGA1961990.12845823257162037No Hit
TGACTTACTATCTGTG1951340.12776094044633543No Hit
CTTTAAGCTCACCAAC1946170.12742244276673703No Hit
CACAGGATGCTTTAGG1940190.12703091160155358No Hit
GTAGTAACTTGTTGGC1917630.12555383081785143No Hit
ATGCTTATGCAAACCT1905440.12475570959651595No Hit
GGATTAGTGGGCGGTA1852880.12131442564299712No Hit
CAATTGGGACCGTTGT1807260.11832752735609592No Hit
GCTCGTTACCGTGCTC1795320.11754577449450887No Hit
CTATCGGCTTGAGCAA1757570.11507415217248958No Hit
CCTCGCAACCTTGCTA1714960.11228432893695996No Hit
AGGCGGTTGTTTCGGA1698230.11118895830259218No Hit
CGTAAAGCTTGGACAA1692040.11078367771522No Hit
TGCGTGTCTCGATTTA1671170.10941724704341756No Hit
CTAACCATGATCAGGA1666760.1091285091774545No Hit
GATATTGGAAGCCATG1648890.10795849882263371No Hit
CTTCATTTGCCGGTAC1629540.10669158777810193No Hit
TCAGTAAACTGGCCAA1599690.10473720562413436No Hit
TTTGCAACTCCTCATT1586330.10386248047917601No Hit
CCCAGTACTGGTCCAC1552240.10163049094387433No Hit
GACTTACCTGTTGCAT1540540.10086445170764582No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)