FastQCFastQC Report
Thu 23 Feb 2023
H5L25AFX5_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5L25AFX5_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences152733691
Sequences flagged as poor quality0
Sequence length8
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGTACT4735238931.003237524064026No Hit
TCATGCGA3797494624.863503102272308No Hit
GGGACTAC3318913721.730069366293257No Hit
CATCAGTG2031267513.29940687415195No Hit
GGGGGGGG51008463.3396992939822296No Hit
ATCGTACA2895400.18957179526290635No Hit
CATCAGTA2260370.147994197298617No Hit
GGACTACA2110220.13816336043368452No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)