FastQCFastQC Report
Thu 23 Feb 2023
H5L25AFX5_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5L25AFX5_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences152733691
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGGACTACATCTCGTATGCCGT4610030.30183451796499833No Hit
GAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGA2345380.1535600943474875No Hit
GAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAA2219430.14531371470620716No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGGACTACATCTCGTATGCCG1738420.11382033581575658No Hit
CTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCT1600160.1047679781404615No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)