FastQCFastQC Report
Thu 16 Mar 2023
H5L23AFX5_n02_37PB.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5L23AFX5_n02_37PB.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11201049
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTAATAAAAACATTGTTTTTAGAGTATAATTTAAAATTAGTTCTGCTC161180.1438972367677349No Hit
CAAAAATTAAAGTTCAACTGTTTAATAAAAACATTGTTTTTAGAGTATAA157880.1409510841350663No Hit
GGATAGTTAAAAGAAAAGAAACTTTAATAAATATTTTTTCCATAGATGAA153870.1373710622996114No Hit
GTTAAAAGAAAAGAAACTTTAATAAATATTTTTTCCATAGATGAATGATT142840.12752377031829787No Hit
GCATAATTTAATTTTGTAGTATATATATAATTGGGATTATGACCTCGATG133080.11881030071379922No Hit
ATTTAAATAGCTGCAGTAAATTGACTGTACAAAGGTAGCATAATCAATAG114660.1023654123823581No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG112710.10062450400850848No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)