FastQCFastQC Report
Thu 16 Mar 2023
H5L23AFX5_n02_37GP.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5L23AFX5_n02_37GP.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10948876
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAAATTAAAGTTCAACTGTTTAATAAAAACATTGTTTTTAGAGTATAA163440.14927559687405356No Hit
GTTTAATAAAAACATTGTTTTTAGAGTATAATTTAAAATTAGTTCTGCTC162910.14879152892041156No Hit
GTTAAAAGAAAAGAAACTTTAATAAATATTTTTTCCATAGATGAATGATT120990.11050449379461416No Hit
ATTTAAATAGCTGCAGTAAATTGACTGTACAAAGGTAGCATAATCAATAG119800.10941762423832364No Hit
GGATAGTTAAAAGAAAAGAAACTTTAATAAATATTTTTTCCATAGATGAA117410.10723475176812669No Hit

[FAIL]Adapter Content

Adapter graph