FastQCFastQC Report
Thu 16 Mar 2023
H5L23AFX5_n02_37BS.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5L23AFX5_n02_37BS.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14025898
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAAATTAAAGTTCAACTGTTTAATAAAAACATTGTTTTTAGAGTATAA246520.1757605823170823No Hit
GGATAGTTAAAAGAAAAGAAACTTTAATAAATATTTTTTCCATAGATGAA220680.1573375194942955No Hit
GTTAAAAGAAAAGAAACTTTAATAAATATTTTTTCCATAGATGAATGATT216900.15464250488631814No Hit
GTTTAATAAAAACATTGTTTTTAGAGTATAATTTAAAATTAGTTCTGCTC192600.13731741097789246No Hit
ATTTAAATAGCTGCAGTAAATTGACTGTACAAAGGTAGCATAATCAATAG172740.12315788978359889No Hit
GCATAATTTAATTTTGTAGTATATATATAATTGGGATTATGACCTCGATG154260.10998226281126527No Hit
TAAAAACATTGTTTTTAGAGTATAATTTAAAATTAGTTCTGCTCAATGAA140470.10015045025994057No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)