FastQCFastQC Report
Thu 16 Mar 2023
H5L23AFX5_n01_37SS.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5L23AFX5_n01_37SS.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12188469
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTG404570.33192848092734206No Hit
CTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTGTAC343890.28214372124997816No Hit
GTCTTTTCGTCCCATAAATTTATTTAAGTTTTCTTACTTAAAAAATAAAT245270.2012311800604325No Hit
CAAATTTATAAAATTTTTTTATAGATAGAAACCGATCTGGCTCACACCGA168980.13863923352473553No Hit
CTTTCGTACAAATTTATAAAATTTTTTTATAGATAGAAACCGATCTGGCT157900.1295486742428438No Hit
ATTTATTTAAGTTTTCTTACTTAAAAAATAAATTTAACTTATAAAATTTA142260.11671687395685218No Hit
CTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTGTACAGTCAAT140440.1152236593455667No Hit
CTTTTCGTCCCATAAATTTATTTAAGTTTTCTTACTTAAAAAATAAATTT132810.1089636442444084No Hit
GCTCCTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTT129940.10660895966507361No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)