FastQCFastQC Report
Thu 16 Mar 2023
H5L23AFX5_n01_37SP.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5L23AFX5_n01_37SP.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13059527
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTG369550.2829734951350076No Hit
CTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTGTAC320580.24547596555372944No Hit
GTCTTTTCGTCCCATAAATTTATTTAAGTTTTCTTACTTAAAAAATAAAT288030.2205516325361554No Hit
CTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTGTACAGTCAAT157050.12025703534285735No Hit
CTTTTCGTCCCATAAATTTATTTAAGTTTTCTTACTTAAAAAATAAATTT153850.11780671688951674No Hit
ATTTATTTAAGTTTTCTTACTTAAAAAATAAATTTAACTTATAAAATTTA147670.11307453937650269No Hit
CTCCCAATAAAATTTTTAATTCATTAATTTTTTTATATAATAAATTAAAA132100.10115220865196727No Hit
CGAGGTCATAATCTCAATTATATATATACTACAAAATTAAATTATGCTGT131830.10094546303246665No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)