FastQCFastQC Report
Thu 16 Mar 2023
H5L23AFX5_n01_37SB.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5L23AFX5_n01_37SB.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15351799
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTG445340.2900897803573379No Hit
CTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTGTAC358950.2338162452491724No Hit
GTCTTTTCGTCCCATAAATTTATTTAAGTTTTCTTACTTAAAAAATAAAT337420.2197918302604144No Hit
CTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTGTACAGTCAAT210090.1368504108215591No Hit
CGAGGTCATAATCCCAATTATATATATACTACAAAATTAAATTATGCTGT164240.10698420426166341No Hit
ATTTATTTAAGTTTTCTTACTTAAAAAATAAATTTAACTTATAAAATTTA162550.10588335608093878No Hit
CTTTTCGTCCCATAAATTTATTTAAGTTTTCTTACTTAAAAAATAAATTT158010.10292604795047147No Hit
GCTCCTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTT154360.10054847643588873No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)