FastQCFastQC Report
Thu 16 Mar 2023
H5L23AFX5_n01_37PS.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5L23AFX5_n01_37PS.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14378447
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTG457600.318254120212009No Hit
CTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTGTAC379630.26402712337431156No Hit
GTCTTTTCGTCCCATAAATTTATTTAAGTTTTCTTACTTAAAAAATAAAT333700.23208347883467528No Hit
CAAATTTATAAAATTTTTTTATAGATAGAAACCGATCTGGCTCACACCGA225510.15683891313157813No Hit
CTTTCGTACAAATTTATAAAATTTTTTTATAGATAGAAACCGATCTGGCT215190.14966150377714646No Hit
CTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTGTACAGTCAAT174740.12152911924354556No Hit
CTCCCAATAAAATTTTTAATTCATTAATTTTTTTATATAATAAATTAAAA167190.11627820445420844No Hit
ATTTATTTAAGTTTTCTTACTTAAAAAATAAATTTAACTTATAAAATTTA165620.11518629237218736No Hit
CTTTTCGTCCCATAAATTTATTTAAGTTTTCTTACTTAAAAAATAAATTT163180.11348930798993799No Hit
CGAGGTCATAATCCCAATTATATATATACTACAAAATTAAATTATGCTGT161680.11244607988609619No Hit
GCTCCTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTT159700.11106901878902499No Hit
TTTTATTATAGATATTAATTTTATTCTTAAAATAAAAAATATTTTTTATA150060.10436453950833495No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)