FastQCFastQC Report
Thu 16 Mar 2023
H5L23AFX5_n01_37PB.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5L23AFX5_n01_37PB.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11201049
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTG257850.23020165343442386No Hit
GTCTTTTCGTCCCATAAATTTATTTAAGTTTTCTTACTTAAAAAATAAAT222390.19854390423611218No Hit
CTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTGTAC203590.18175976196515164No Hit
TTTTATTATAGATATTAATTTTATTCTTAAAATAAAAAATATTTTTTATA184480.1646988599014253No Hit
CTTTCGTACAAATTTATAAAATTTTTTTATAGATAGAAACCGATCTGGCT175910.15704779079173745No Hit
CAAATTTATAAAATTTTTTTATAGATAGAAACCGATCTGGCTCACACCGA174970.1562085836781894No Hit
ATAGATATTAATTTTATTCTTAAAATAAAAAATATTTTTTATAAACAATC146280.13059491124447362No Hit
TATAGATATTAATTTTATTCTTAAAATAAAAAATATTTTTTATAAACAAT130700.11668549972417763No Hit
CTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTGTACAGTCAAT126860.11325724938798143No Hit
CGAGGTCATAATCCCAATTATATATATACTACAAAATTAAATTATGCTGT117900.10525799860352364No Hit
ATTTATTTAAGTTTTCTTACTTAAAAAATAAATTTAACTTATAAAATTTA115310.10294571517364133No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)